Figure 2

Definition of the regions of interest. (a) Schematic top view of enzyme diffusion (maroon) inside the gel sample (black) and definition of the regions of interest, referred to as Pos for Position (white squares). Three time points are represented: at T0, the channel is full of enzyme (maroon); at T1, the enzyme diffuses into the gel according to the white arrows; at Tn, the channel is empty. (b) From the deposit point of the enzyme (right side of the gel), the 2 first Pos corresponded to the two first fields at magnification x40, the next ones corresponded to 1 field over 2. The 7 positions were numbered from Pos0 to Pos6. Values below the positions are the distance from the deposit point.