Table 1 Markers used for foreground selection of Sub1QTL and four bacterial blight resistance genes in marker-assisted backcross breeding.
Resistance gene | Chromosome number | Marker | Primer sequences used for gene detection | Expected size (bp) | Marker type | Reference | |
|---|---|---|---|---|---|---|---|
Forward(5′-3′) | Reverse(5′-3′) | ||||||
xa5 | 5 | RM122 | GAGTCGATGTAATGTCATCAGTGC | GAAGGAGGTATCGCTTTGTTGGAC | 260 bp | SSR | |
xa5S (Multiplex) xa5SR/R (Multiplex) | GTCTGGAATTTGCTCGCGTTCG AGCTCGCCATTCAAGTTCTTGAG | TGGTAAAGTAGATACCTTATCAAACTGGA TGACTTGGTTCTCCAAGGCTT | 160 bp | STS | |||
xa13 | 8 | Xa-13 prom | TCCCAGAAAGCTACTACAGC | GCAGACTCCAGTTTGACTTC | 500 bp | STS | |
Xa21 | 11 | pTA248 | AGACGCGGAAGGGTGGTTCCCGGA | AGACGCGGTAATCGAAGATGAAA | 1000 bp | STS | |
Xa4 | 11 | MP-Nbp-131 | ATCGATCGATCTTCACGAGG | TGCTATAAAAGGCATTCGGG- | 250 bp | STS | |
Sub1 | 9 | RM8300 | GCTAGTGCAGGGTTGACACA | CTCTGGCCGTTTCATGGTAT | 205 bp | SSR | |
Sub1-A203 | CTTCTTGCTCAACGACAACG | AGGCTCCAGATGTCCATGTC | 200 bp | STS | |||
Sub1-BC2 | AAAACAATGGTTCCATACGAGAC | GCCTATCAATGCGTGCTCTT | 240 bp | STS | |||
Sub1-C173 | AACGCCAAGACCAACTTCC | AGGAGGCTGTCCATCAGGT | 170 bp | STS | |||
