Table 3 Primers used for qPCR experiments to characterise isoforms of endocannabinoid receptors. An asterisk (*) indicates that the primer spans an isoform-specific exon-exon boundary. The two nucleotides involved in the isoform-specific exon junction are underlined.
From: Characterisation and localisation of the endocannabinoid system components in the adult human testis
Target | Primer sequence (5′ → 3′) | Amplicon size | Annealing temperature |
|---|---|---|---|
CNR1 (CB1) | F: TCAGTACGAAGACATCAAAGGTG | 85 bp | 60 °C |
R: CTTCCCCTAAAGGAAGTTAAAGG | |||
CB1A | F: AGACATCAAAGGAGAATGAGGAG* | 113 bp | 60 °C |
R: GAGGGACAGGACTGCAATG | |||
CB1B | F: ACTGACCTCCTGGGAAGTCC * | 84 bp | 64 °C |
R: AATGTTCACCTGGTCTGCTG | |||
CB2A | F: GATTATGCCAGCCAGATGC | 77 bp | 64 °C |
R: GCTCGGTGAGTGAGAGGTG |
