Figure 4 | Scientific Reports

Figure 4

From: Human multipotent hematopoietic progenitor cell expansion is neither supported in endothelial and endothelial/mesenchymal co-cultures nor in NSG mice

Figure 4

HSPC repopulation assay in immunodeficient mice. (A) Experimental design and gating strategy. UCB-derived CD34+ cells were purified, phenotypically characterized by flow cytometry and transplanted into NSG mice. Murine BM was harvested 8 weeks post-transplant (TX) and analyzed for HSPC content. (B) Bar graphs reflect human CD45+ cell engraftment in the BM and PB as well as frequency of CD34+ cells in the BM of transplanted mice. (C) Representative flow cytometric assessment of phenotypically defined HSPC subsets in freshly-isolated UCB-derived CD34+ cells and engrafted human CD34+ cells 8 weeks post-transplant. (D) Quantitative comparison of HSPC subsets pre- and post-transplantation. Bar graphs: Mean ± SD. Statistical analysis: Two-way ANOVA (n = 7).

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