Figure 3
AOX expression modifies the cardiac phenotype of Mcp1 overexpressing mice. (A) Survival curves of mice of the indicated genotypes (wt – wild-type, Mcp1 – overexpressing Mcp1 in cardiomyocytes under the control of the Myh6 promoter; global AOX – expressing AOX constitutively; cardio AOX – expressing AOX specifically in cardiomyocytes, after activation of SNAPf-AOX by Myh6-cre, Myh6-cre – expressing only cre in adult cardiomyocytes, and combinations as shown, n ≥ 10 for all groups except those including Myh6-cre, where n ≥ 5). The survival curve for Mcp1 plus cardiomyocyte-specific AOX was significantly different from that of Mcp1 alone, or together with global AOX (p < 0.0001, Log-rank (Mantel-Cox) or Gehan-Breslow-Wilcoxon test, see Table S2). (B) Systolic and diastolic volume of the cardiac left ventricle (LV) of mice of the indicated genotypes, measured by ultrasound at 12 and 16 weeks, as indicated (means ± SD, n ≥ 5 for all groups). (C) Cardiac ejection fraction (EF, %) computed for individual mice of the indicated genotypes and ages, means ± SD. Horizontal lines marked with asterisks indicate significant differences (one-way ANOVA within an age point, applying Tukey’s post hoc HSD test; *, **, ***p < 0.05, 0.01, 0.001, respectively).
