Table 2 Samples collected by ultrasound-guided fine-needle aspiration (US-FNA).

From: Suitability of ultrasound-guided fine-needle aspiration biopsy for transcriptome sequencing of the canine prostate

Dog ID

Sample ID

Reproductive status

Number of US-FNA procedures

Cellularity of smears

Diagnosis

Blood Scoring

RNA (ng/µl)

RIN

RNA-Seq

1*

US-1

m

1

S

PCa

N/A

2**

US-2

n

3

S

PCa

N/A

US-3

4

59

N/A

y

3

US-4

n

1

S

PCa

2

44.1

6.9

y

4

US-5

m

1

S

nm

0

2.3

5

US-6

n

2

S

PCa

1

9.1

N/A

6

US-7

m

2

S

nm

3

35.6

6.2

y

7

US-8

m

3

S

nm

4

37.5

8

US-9

m

2

S

nm

4

91

9

US-10

n

2

S

PCa

3

21.7

1.9

10

US-11

m

1

S

nm

3

33.2

11

US-12

m

1

S

nm

4

85

12

US-13

m

1

S

nm

2

90

6

y

13

US-14

m

1

S

nm

3

54

14

US-15

m

3

S

nm

4

61

6.1

y

15

US-16

m

1

S

nm

3

74

5.7

16

US-17

m

1

Nd

N/A

3

69

17

US-18

m

1

S

nm

2

66

18

US-19

m

2

S

nm

3

20.9

19

US-20

m

2

S

nm

3

41.1

20

US-21

m

1

S

nm

2

11.2

1

21

US-22

m

2

S

nm

2

8.2

22

US-23

m

1

Nd

N/A

3

18.7

23

US-24

m

1

S

nm

0

26.6

24

US-25

m

1

S

nm

3

54

25

US-26

m

1

S

nm

2

30.1

4.2

26

US-27

m

1

S

nm

3

56

27

US-28

m

1

S

nm

4

47.8

N/A

28

US-29

m

1

S

nm

2

30

29

US-30

m

1

S

nm

3

72

30

US-31

m

1

Nd

N/A

0

N/A

31

US-32

m

1

S

nm

3

60

6.7

y

32

US-33

n

1

Nd

N/A

2

11.3

1.5

33

US-34

m

1

S

nm

2

51

34

US-35

m

1

S

nm

2

68

35

US-36

m

1

S

nm

3

378.6

36

US-37

m

1

S

nm

3

48

37

US-38

m

1

S

nm

3

24

3.2

38

US-39

m

1

S

nm

3

99

5.7

39

US-40

m

2

S

nm

4

601.1

7.2

y

40

US-41

n

1

Nd

N/A

3

98

41

US-42

m

3

S

nm

3

72

42***

N/A

m

43***

N/A

m

  1. Sample details, including: identification number (ID) of the dogs and the corresponding sample ID; reproductive status, male (m) and neutered (n); cytological smears, sufficient (S) and non-diagnostic (Nd); diagnosis, prostate carcinoma (PCa) and non-malignant (nm); semi-quantitative scoring of blood contamination as follows: 0 = no, 1 = minimal, 2 = weak, 3 = moderate, 4 = strong blood contamination; RNA quantity; RNA integrity number (RIN); and samples used for RNA-Seq.
  2. *Dog euthanised due to prostate carcinoma enabled additional sampling opportunity.
  3. **Dog euthanised due to prostate carcinoma enabled additional sampling including one additional ultrasound-guided fine-needle aspiration.
  4. ***US-FNA procedure was omitted.
  5. N/A, not available.
  6. y, US-FNA samples selected for RNA-Seq.