Figure 1
From: Role of POMC and AgRP neuronal activities on glycaemia in mice
: Stimulation of AgRP neuronal activity in lean normoglycaemic mice affects food intake and insulin sensitivity but not blood glucose levels. Food intake (daily (a) and acute (c)), blood glucose (daily (b) and acute (d)), body weight (e), GTT (f), and ITT (g) of AgRP-ires-cre male normoglycaemic mice. (h) Representative m-Cherry expression image for the verification of the injection site. Data are shown as means ± s.e.m. (n = 4–6/group). 2-way repeated measures ANOVA was first done to determine the intervention effect or intervention-by-time interactions for all studies except area under the curve (AUC). Then, when intervention differences and/or intervention-by-time interactions were significant by 2-way repeated-measures ANOVA, GLM procedures or 1-way ANCOVA were performed to evaluate the effect of CNO on dependent variables where appropriate. Body weight was used as the covariate while performing 1-way ANCOVA. Logarithmic transformation was done for daily food intake values to normalize variance. AUC or AAC was evaluated with 1-way ANCOVA. CNO (1 mg/kg) was injected every 8 hours daily. AAV8-DIO-hM3Dq-mCherry (activator) was injected into the study and control mice that received CNO and PBS, respectively for 5 days. All mice then received PBS for an additional 3 days. CNO: Clozapine N-oxide. GTT: Glucose tolerance test. ITT: Insulin tolerance test. 3V: Third ventricle. ARC: Arcuate nucleus. The image in (h) was captured at 20× magnification (cropped upper side of slide). Scale bar, 100 μm. *P ≤ 0.05, ***P ≤ 0.001, #P ≤ 0.05.
