Figure 3
From: Role of POMC and AgRP neuronal activities on glycaemia in mice
Stimulation of AgRP neuronal activity in ob/ob mice is sufficient to attenuate the effect of leptin on food intake, but not on blood glucose. Food intake (daily (a) and acute (c)), blood glucose (daily (b) and acute (d)), body weight (e) of diabetic ob/ob; AgRP-ires-cre male mice that were implanted with an osmotic mini-pump loaded with leptin (500 ng/h for 13 days). (f) Representative m-Cherry expression image for the verification of the injection site. Data are shown as means ± s.e.m. (n = 4/group). Statistics were performed as described in Fig. 1. P -value (interaction) in (e) was obtained by Greenhouse-Geisser correction. CNO administration at day 9 led to an increase in body weight of mice infused with leptin at day 10 (P = 0.007, paired t-test). AAV8-DIO-hM3Dq-mCherry (activator) was injected into the leptin- or PBS-injected ob/ob mice. CNO (1 mg/kg) or PBS was injected 5 times a day at day 9 or before the acute experiments ((c) and (d)). Abbreviations are the same as described in Fig. 1. The image in (f) was captured at 20× magnification accompanied by another image at higher magnification. Scale bars, 60 μm. *P ≤ 0.05 [different from other group(s)], **P ≤ 0.01 [different from other group(s)]. P ≤ 0.01 between day 9 and day 10.
