Figure 3
Biomarker expression was assessed by immunocytochemistry for PA5 c-MycERTAM-derived hOMCs at passage 8, and primary cells (phOMCs) at passage 3. Both cell populations stained positive for OEC markers (p75NTR, S100ß and oligodendrocyte marker O4), neural markers (nestin and ß-III-tubulin), and fibroblast-associated markers (fibronectin, CD90/Thy1) for PA5 and primary hOMCs. Primary hOMCs were negative for GFAP, whereas PA5 hOMCs were positive for this glial marker. Scale bars represent 200 µm.
