Figure 4
From: Dynamic Remodeling of the Host Cell Membrane by Virulent Mycobacterial Sulfoglycolipid-1
Nanomechanical membrane properties of live THP-1 cells upon lipid perturbation. (A) Elastic moduli distribution of THP-1 cells in the absence (n = 199 cells median: 1.74 kPa) and presence of 4.16 μM SL-1 (n = 210 cells, median: 1.44 kPa) at 2 h, fitted with a single log-normal distribution. (B) Relative frequency of membrane tether forces in control (n = 1182 elements, median 85.7 pN) and SL-1 treated (n = 1134 elements, median 74.4 pN) cells fitted with a log-normal distribution. (C) Tether number distribution of THP-1 cells in the absence (n = 169 cells, median: 5.0) and presence of 4.16 μM SL-1 (n = 184 cells, median: 7.0) fitted with a single log-normal distribution. The medians of the data in A-C were significantly different from each other (P < 0.05, Mann-Whitney test). (D) Tether length distribution of control and SL-1 treated cells (n = 175 each, mean + SEM: 6.2 ± 0.1 μm and 5.2 ± 0.2 μm, respectively) with significant mean difference (***P < 0.0001, unpaired student t-test).
