Figure 5

8-Isoprostane levels in non-stimulated and LPS-stimulated U937 cells incubated in the presence and/or absence of DHA (20 μM; 80 μM), the FFA4 antagonist AH7614 and the HO-1 inhibitor SnPP. Supplementation of cells with DHA 80 μM decreased 8-isoprostane production (A), a finding that was not reversed with AH7614 treatment (B). In LPS-stimulated cells treated with SnPP (C), no significant effect was observed for DHA at either of the concentrations tested. Data are expressed as mean ± SEM and are representative of at least six independent experiments. *p < 0.05.