Figure 6
Comparison of the localization of Bax expressed in cells or transferred by osmotic shock. (a) HeLa cells transfected with plasmids for the mitochondrial marker DsRed-Mito (magenta) and GFP-Bax (green) before and after pro-apoptotic stimuli (1 µM Staurosporin, STS). (b) HeLa cells after osmotic shock, showing the uptake of BaxC4Atto488 (green). To visualize the mitochondria and their integrity, TMRE (magenta) was added as mitochondrial marker. Upper row: overview images; lower row: enlarged sections. Scalebar: 20 µm. Additional control experiments are given in Supp. Fig. 13.
