Figure 3

Determination of pyoverdine (A), pyocyanin (B), protease (C), and rhamnolipid (D) production by unevolved and evolved P. aeruginosa AA2 populations. Graphs show means, error bars indicate standard deviations. n ≥ 3, *p ≤ 0.05. T0: timepoint 0, planktonic culture. T1: timepoint 1, 72 h biofilm cells. T18: timepoint 18, biofilm cells after 18 cycles (54 days). L1: lineage 1, L2: lineage 2, L3: lineage 3. C: control single-culture, M: microbiome.