Figure 1

Overview of the key benefits of induced morphology changes of cells and organelles. Graphical illustrations are shown pre (top row) and post (bottom row) swelling induction. (a) Non-isotropic swelling enlarges otherwise non-resolvable biological structures for improved resolution, for example, of membrane (dark gray) vs. lumen (light gray) protein locations, as shown in Figs 4, 5. (b) Non-isotropic swelling leads to morphological changes for improved separation and quantification of objects, as shown in Fig. 7b,c. (c) Swelling enlarges the volume of cells for improved separation and quantification of clumped intracellular objects, as shown in Fig. 7a. (d) Swelling of cells is inducible and reversible for transient analyses, as shown in Fig. 6 and Fig. 7c.