Figure 4

Improved protein location determination upon organelle swelling. (a,b) MIP images (columns 1–3) and single slice images (column 4) of (a) a U2-OS cell co-expressing CD3δ-GFP (green) and ssRFP-KDEL (red) and (b) a COS-7 cell co-expressing TOMM20-GFP (green) and mCherry-Mito (red). The single slice images (column 4) are 2.6x magnifications (magn.) of the corresponding area outlined in column 3 (merge, yellow rectangle). Profiles of fluorescence intensity (arbitrary units) taken along the white dotted lines (column 4) are presented (right). ER (a) and mitochondria (b) signals are shown pre (culture medium, top rows) and post incubation in diH₂O for 200 s (diH₂O, bottom rows). Scale bars, 5 µm (column 3), 1 µm (column 4).