Figure 6

Inducible and reversible cell and organelle swelling. (Top row) Side views of 3D volume renderings of a N2a cell co-expressing the plasma membrane (PM) marker MyrPalm-mEGFP (green) and H2B-mCherry (red). (Bottom row) MIP images of a COS-7 cell co-expressing GFP-Mito (green) and ssRFP-KDEL (red). Shown are cells pre (culture medium, left column) and post incubation in diH₂O at time points 1 min (column 2) and 3 min (column 3) and upon re-suspension in cell culture medium at 5 min (right column). Scale bars, 10 µm.