Table 1 Cell survival upon diH₂O incubation. Shown are the cell survival rates of different cell lines upon incubation in diH₂O over time (3.3, 5, 15, 30, 60 min).

Cell line	Incubation in diH₂O prior to microscopy
Cell line	0 min	3.3 min^#	5 min	15 min	30 min	60 min
HeLa	+++	+++	+++	+++	+++	+++*
N2a*	+++	+++	+++	+++	+++	+++
COS-7	+++	+++	++++*	− +++*	− +++*	n.t. +++*
U-2 OS	+++	+++	+++	+++	+++	+++*
RPE-1	+++	+++	+++	+++	+++	+++*
HEK 293	+++	+++	** +++*	** +++*	** +++*	** +++*

Cell survival of the cell population is indicated: +++ is>= 95%, +is 25–50%, −is no survival. *Swelling performed with 4:1 (v/v) mixtures of diH₂O and cell culture medium. **HEK 293 cells round up and detach, which prevents change of media prior to microscopy. n.t., not tested. All cell survival analyses for all cell lines are based on 3 independent replicates for each condition. As example, one corresponding image series for each condition is shown in Supplementary Video 8, except as indicated (^#) in Supplementary Video 7.

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