Figure 1

Delivery of hMSC after TBI. (A) Biodistribution of hMSCs after TBI. Representative Xenogen images showing DiR fluorescence in different organs 7 days after TBI. (B) Histogram showing the significantly higher level of DiR fluorescence in brain and spleen following intra-nasal (i.n.) administration as compared to intra-venous (i.v.) administration, n = 3 animals/group. *p < 0.01 vs i.v. (C) Brain sections from rats after 35 days of TBI were stained with anti-human nuclear antigen (HuNu) antibody (clone 235-1). The immunostaining indicates the presence of hMSCs (arrows) in the ipsilateral cortex close to the lesion site. Scale bar 100 µ, inset scale bar 20 µ. (D) Histogram showing the percentage of HuNu positive cells in the perilesional area of the brains. HuNu positive cells which were also DAPI positive were counted from the perilesional area of each section located 120 µ apart. Also, 50 DAPI positive cells were counted from the same area of the sections. % of HuNu positive cells was calculated from 7 dpi and 35 dpi brains as shown in the histogram. A paired t test did not show any significant difference between the % of HuNu positive cells present in the brains of 7 dpi or 35 dpi. n = 3 (7 dpi), and 7 (35 dpi).