Table 2 Validation of RT-qPCR assay parameters for primers targeting aiiO, gyrB, rho and rpoD.

From: Selection of reference genes for measuring the expression of aiiO in Ochrobactrum quorumnocens A44 using RT-qPCR

Parametera

aiiO b

gyrB

rho

rpoD

Efficiency (%)

94.9

95.9

93.5

89

R2

0.999

1

1

0.999

Slope

−3.451

−3.425

−3.487

−3.618

  1. aEfficiency – calculated primer efficiency; R2 – correlation between log Cq and Cq in the standard curve; Slope – slope of the standard curve.
  2. bPrimers used for PCR amplification: aiiO: F_aiiO_OP7/R_aiiO_OP7; gyrB: F_ gyrB_OP7/R_ gyrB_OP7; rho: F_ rho_OP7/R_ rho_OP7 (Table S1).
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