Table 2 Expression stability ranking of the twelve candidate reference genes.
Experimental conditions | Methods | Stability ranking | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||
Tissues | geNorm | RPL13A/RPS3A | Actin | UBQ | EF1α | EIF4A | GAPDH | TBP | 18SRNA | β-Tubulin2 | G6PDH | β-Tubulin1 | |
NormFinder | RPS3A | Actin | UBQ | EF1α | RPL13A | GAPDH | EIF4A | TBP | β-Tubulin2 | 18SRNA | G6PDH | β-Tubulin1 | |
Delta Ct | RPS3A | Actin | UBQ | EF1α | RPL13A | EIF4A | GAPDH | TBP | β-Tubulin2 | 18SRNA | G6PDH | β-Tubulin1 | |
Bestkeeper | G6PDH | Actin | RPL13A | UBQ | RPS3A | EIF4A | 18SRNA | GAPDH | EF1α | TBP | β-Tubulin2 | β-Tubulin1 | |
RefFinder | RPS3A | Actin | RPL13A | UBQ | EF1α | G6PDH | EIF4A | GAPDH | TBP | 18SRNA | β-Tubulin2 | β-Tubulin1 | |
Developmental stages | geNorm | EF1α/EIF4A | GAPDH | RPS3A | RPL13A | G6PDH | β-Tubulin1 | UBQ | 18SRNA | TBP | Actin | β-Tubulin2 | |
NormFinder | RPL13A | GAPDH | β-Tubulin1 | EIF4A | RPS3A | EF1α | UBQ | 18SRNA | G6PDH | TBP | Actin | β-Tubulin2 | |
Delta Ct | RPL13A | GAPDH | EIF4A | EF1α | RPS3A | β-Tubulin1 | UBQ | 18SRNA | G6PDH | TBP | Actin | β-Tubulin2 | |
Bestkeeper | UBQ | 18SRNA | β-Tubulin1 | RPL13A | Actin | β-Tubulin2 | GAPDH | TBP | G6PDH | RPS3A | EIF4A | EF1α | |
RefFinder | RPL13A | GAPDH | EIF4A | EF1α | UBQ | β-Tubulin1 | 18SRNA | RPS3A | G6PDH | Actin | TBP | β-Tubulin2 | |
Sexes | geNorm | RPL13A/RPS3A | EIF4A | EF1α | UBQ | β-Tubulin1 | 18SRNA | GAPDH | Actin | G6PDH | TBP | β-Tubulin2 | |
NormFinder | EF1α | Actin | RPL13A | EIF4A | RPS3A | GAPDH | UBQ | 18SRNA | G6PDH | β-Tubulin1 | TBP | β-Tubulin2 | |
Delta Ct | EF1α | RPL13A | EIF4A | RPS3A | UBQ | GAPDH | Actin | 18SRNA | β-Tubulin1 | G6PDH | TBP | β-Tubulin2 | |
Bestkeeper | β-Tubulin1 | UBQ | EIF4A | RPS3A | RPL13A | GAPDH | EF1α | G6PDH | 18SRNA | Actin | TBP | β-Tubulin2 | |
RefFinder | EF1α | RPL13A | RPS3A | EIF4A | UBQ | β-Tubulin1 | Actin | GAPDH | 18SRNA | G6PDH | TBP | β-Tubulin2 | |
All samples | geNorm | RPL13A/RPS3A | EIF4A | EF1α | GAPDH | UBQ | 18SRNA | TBP | G6PDH | β-Tubulin1 | β-Tubulin2 | Actin | |
NormFinder | RPL13A | EIF4A | RPS3A | EF1α | 18SRNA | UBQ | GAPDH | TBP | β-Tubulin1 | G6PDH | β-Tubulin2 | Actin | |
Delta Ct | RPL13A | EIF4A | RPS3A | EF1α | UBQ | GAPDH | 18SRNA | TBP | β-Tubulin1 | G6PDH | β-Tubulin2 | Actin | |
Bestkeeper | RPL13A | β-Tubulin1 | RPS3A | EIF4A | 18SRNA | Actin | TBP | UBQ | EF1α | GAPDH | β-Tubulin2 | G6PDH | |
RefFinder | RPL13A | RPS3A | EIF4A | EF1α | 18SRNA | UBQ | β-Tubulin1 | GAPDH | TBP | Actin | G6PDH | β-Tubulin2 | |
