Figure 3
From: The double inhibition of PDK1 and STAT3-Y705 prevents liver metastasis in colorectal cancer
Knockdown of PDK1 and inhibiting p-STAT3-Y705 decreased liver metastasis of colon cancer in the nude mice model. (A) 5 × 106 HCT116 cells with or without PDK1 shRNA transduction were injected into the spleens of nude mice under isoflurane anesthesia on day 1, respectively. On day 9, the nude mice were sacrificed, and the liver metastatic area was measured by ImageJ software. Compared with the control group, knockdown of PDK1 significantly inhibited liver metastasis of HCT116 cells. (B) SH-4-54 (an inhibitor of total STAT3, 0.2 mg/d/mouse) or CPT (0.2 mg/d/mouse) were injected (i.p.) every other day (day 1, 3, 5, 7) for a total of four times. The results showed that CPT plus knockdown of PDK1 markedly decreased the liver metastasis area, compared with the combination of SH-4-54 and knockdown of PDK1. (C) Histogram indicated the corresponding comparison of liver metastasis areas presented in (A,B). Data expressed as mean ± S.D., * represents P < 0.05, ** represents P < 0.01.
