Figure 4
From: The double inhibition of PDK1 and STAT3-Y705 prevents liver metastasis in colorectal cancer
The combination of CPT and silencing PDK1 significantly inhibited liver metastasis of CRC by down-regulating p-STAT3-Y705 in the immune competent mice model. (A) 5 × 106 HCT116 cells with or without PDK1 shRNA transduction were injected into the spleens of BALB/C mice under isoflurane anesthesia, respectively. CPT (0.2 mg/d/mouse) were injected (i.p.) every other day (day 1, 3, 5, 7) for a total of four times. On day 9, the mice were sacrificed, and the liver metastatic area was measured by ImageJ software. The combination of CPT and PDK1 shRNA treatment led to a smallest liver metastasis area, compared with the control or PDK1 shRNA treatment alone. (B) Histogram indicated the corresponding comparison of liver metastasis areas presented in (A). Data expressed as mean ± S.D., **** represents P < 0.0001.
