Figure 6
From: The double inhibition of PDK1 and STAT3-Y705 prevents liver metastasis in colorectal cancer
Anoikis partly contributes to the in vivo effect of the combination of knockdown of PDK1 and CPT. (A) HCT116 cells with or without the transduction of a PDK1 shRNA were seeded into 6-well plates and cultured in normal conditions. Then the cells were stained with AnnexinV-FITC/PI. Flow cytometry showed that the apoptosis rate was much higher after PDK1 was silenced. (B) As indicated in (A), the cells were seeded into 6-well plates coated with poly-HEMA. Flow cytometry showed that the apoptotic rate of HCT116 cells stably transduced with PDK1 was significantly higher than the other two controls. (C) Histogram indicated the corresponding comparison of the apoptosis rate presented in (A,B). (D) The results showed knockdown of PDK1 strongly sensitized CRC to anoikis; however, the anoikis rate was significantly reduced when PDK1 silencing was combined with CPT. (E) Histogram indicated the corresponding comparison of the apoptosis results presented in both normal condition and suspending condition in (D). Data expressed as mean ± S.D., *** represents P < 0.001, and **** represents P < 0.0001.
