Figure 1 | Scientific Reports

Figure 1

From: Simple 3D culture of dissociated kidney mesenchyme mimics nephron progenitor niche and facilitates nephrogenesis Wnt-independently

Figure 1

Schematic representation of the 3D culture method of dissociated kidney mesenchyme. (A) E11.5 embryonic mouse kidneys are isolated and subjected to collagenase IV treatment after which the KM is separated from the epithelial ureteric bud (UB) by manual microdissection. (B) Dissociation of KM into single cells assisted by TrypLE enzymatic digestion, resulting in single cell suspension (dissociated KM). (C) After pelleting the cells with centrifugation, the dissociated KM is resuspended into Basic Media (BM). Dissociated KM cells are then plated on top of the 3D Matrigel submerged in either (D) BM, (E) BM + 50 ng/ml FGF2 or (F) BM + 50 ng/ml FGF2 and pulsed with 10 µM BIO for 1 hour. In some experiments BIO pulse is replaced by constant BIO application at 50 nM concentration.

Back to article page