Figure 3
CaSr-MOFs induce differentiation of pre-osteoblastic cells to osteoblastic cells, and induce bone mineralization. (A) A brightfield image of CaSr-MOFs with MC3T3 cells. (B) Assay for determining %alive MC3T3 cells in the presence of different MOFs. (C) Alkaline phosphatase generation by MC3T3 in the presence of different MOFs for day 3 and day 7 culture. The no treatment group is the negative control for the corresponding concentration of MOFs. (D) Bone mineralization (shown in green) by MC3T3 in the presence of CaSr-MOF compared to positive (osteogenic medium) and negative (no treatment) controls. (E) Quantification of bone mineralization for treatment with different MOFs.
