Table 1 Primer sequences used for qRT-PCR, along with accession numbers in the NCBI nucleotide database.
Gene | Forward Primer Sequence (5′-3′) | Reverse Primer Sequence (5′-3′) | Product Size (bp) | Accession Number |
|---|---|---|---|---|
18S | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG | 151 | NR_146146.1 |
ALP | ATCTTTGGTCTGGCCCCCATG | AGTCCACCATGGAGACATTCTCTC | 124 | NM_000478.6 |
BSP II | CGAATACACGGGCGTCAATG | GTAGCTGTACTCATCTTCATAGGC | 109 | NM_004967.4 |
RUNX2 | CAACCACAGAACCACAAGTGC | TGTTTGATGCCATAGTCCCTC | 196 | NM_001024630.4 |
OCN | ATGAGAGCCCTCACACTCCTC | GCCGTAGAAGCGCCGATAGGC | 294 | NM_199173.6 |
OPN | TCACCAGTCTGATGAGTCTCACCATTC | TAGCATCAGGGTACTGGATGTCAGGT | 203 | NM_001040058.2 |
COL1A1 | TAAAGGGTCACCGTGGCT | CGAACCACATTGGCATCA | 355 | NM_000088.3 |
