Figure 1

The Puerto Rican fireworm. (a) Bright field image of anterior part. (b) Luminous mucus from a worm that was pressed on paper. The bluish-green color appeared similar to that observed in their natural secretion during the mating behavior on the sea surface. Scale bar = 2 mm. (c) i to v are a series of assays using different combination of substrate extract (PRs and JPs) using the native PRe. A 10 μL of PRe was measured in 100 μL RB in total (i) and left for 3 hours (ii). Then, 1 μL JPs was added to this solution to check the cross reaction (iii). The activity went down to almost background level after 1 hour of the reaction (iv) and 10 μL PRs was added to the mixture (v). The activity using 1 μL JPre and 10 μL PRs is shown in (vi).