Figure 3

BN-PAGE analysis of PSII supercomplex organization and abundance as a function of Chl b abundance. Thylakoid membranes were isolated from WT (Chl a/b was 3.4), CR L-I (Chl a/b was 4.6), CR H-I (Chl a/b was 7.9) and CR V-H (Chl a/b was 10.0) lines. (a) An aliquot of thylakoid suspension containing 8 μg of Chl was solubilized with final concentration of 1% (w/v) β-DM and was loaded on a 4.5–13.5% BN-PAGE. Identities of the photosystem complexes are given on the left of the panel. The PSII supercomplexes are assigned as follows: SC1 (C₂S₂M₂), SC₂ (C₂S₂M), SC3 (C₂S₂) and SC4 (C₂S). The identification of all other bands was made according to Rantala et al.¹⁷. (b) CP complexes isolated by sucrose density gradient ultracentrifugation. Thylakoid membranes were isolated from mixed leaves from 6 plants of each WT (Chl a/b was 3.5), and CR L-I (Chl a/b was 5.2), CR H-I (Chl a/b was 8.0) and CR V-H (Chl a/b was 9.8) lines. The identification of all bands was made according to Barera et al.²¹. (c) SDS-PAGE analysis of LHCII trimer. M-Molecular weight markers, 1-Total thylakoids isolated from WT plants, 2-LHCII trimer excised from BN-gel (Fig. 3a, WT), 3-LHCII trimer fraction from SDGU (WT), 4- LHCII trimer fraction from SDGU (CR L-I), 5- LHCII trimer fraction from SDGU (CR H-I), 6- LHCII trimer fraction from SDGU (CR V-H). Chl content was determined according to Porra et al.³⁴.