Figure 4

Quantification of haemostatic control following BECA and challenges and treatment with purified prothrombin. Panel (A), blood loss in µl following tail transection (TT) and treatment with 12 ml/kg body weight of vehicle (Vehicle, 5% HAS, grey bar), PCC (hatched bar), or purified human prothrombin (black bars) containing the dose in IU/kg specified below the axis. Values are presented as mean ± SD, n = 15 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001 versus groups linked by horizontal capped lines by ANOVA with post-tests. Panel (B), blood loss as clot weight in mg following liver laceration (LL) following treatment with 12 ml/kg vehicle (Vehicle, 5% HAS, grey bar) or 36 IU/kg FIX (white bar) or 36 IU/kg purified human prothrombin (black bar). Values are presented as in A but for n = 7. Panel (C) shows kinetic curves of platelet mean fluorescent intensity (MFI) detected by fluorescent intravital microscopy and measured every 15 seconds following laser injury of cremaster muscle arterioles, for mice receiving 36 IU/kg PCC or FIX. Values are presented as in B but for n = 5 or 6 thrombi; only the upper error bar (SD) is shown for clarity. In Panel (D), the curves shown in Panel (C) were quantified as the area under the platelet MFI versus time curve (AUC).