Figure 5

Quantification of haemostatic control following BECA with challenges and treatment with murine plasma. Panel (A), blood loss in µl following tail transection (TT) and treatment with 12 ml/kg body weight of plasma from donor mice treated with ASO-CON (ASO-CON mFFP, grey bar) or ASO-FII (ASO-FII mFFP, black bar). Values are presented as mean ± SD, n = 15 mice per group; *p < 0.05, **p < 0.01, ***p < 0.001 versus groups linked by horizontal capped lines by Welch-corrected t test. Panel (B), blood loss as clot weight in mg following liver laceration (LL) following treatment with ASO-CON (grey bar) or ASO-FII (black bar) mFFP. Values are presented as in A but for n = 7. Panel (C) shows kinetic curves of platelet mean fluorescent intensity (MFI) detected by fluorescent intravital microscopy and measured every second following laser injury of cremaster muscle arterioles, for mice receiving ASO-CON (grey line) or ASO-FII (black line) mFFP. Values are presented as in B but for n = 5 or 6 thrombi; only the upper error bar (SD) is shown for clarity. In Panel (D), the curves shown in Panel (C) were quantified as the area under the platelet MFI versus time curve (AUC).