Figure 1
From: Identification of transcriptome-wide, nut weight-associated SNPs in Castanea crenata
Overview of the systematic SNP analysis. A total of 21 SNPs (FDR < 10−5) related to chestnut weight were obtained and validated, as shown above. Individual cut-off values and bioinformatic programs are denoted parallel to each step. Illumina RNA sequencing was performed using 42 chestnut trees, categorised as either large or small nut-bearing populations. For the machine learning algorithms, 70% of the 42 chestnut tree genotypes were randomly used for training and the remaining 30% were used for testing. For further validation, using Sanger sequencing, another 46 chestnut tree genotypes were used as the validation set.
