Figure 8 | Scientific Reports

Figure 8

From: Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules

Figure 8

Analysis of current-blockade events derived from SA-labelled and non-labelled dsDNA passing through nanopores at various mixing ratios of SA-labelled and non-labelled dsDNA. (a) Time traces of ionic current through nanopores fabricated in 14-nm-thick SiN membranes, scatter plots of current-blockade events and histograms of ΔImax. The voltage applied was 0.2 V (Vtrans = 0.2 V and Vcis = 0 V). Among nine experiments, the results for five different mixing ratios of SA-labelled 400-bp dsDNA and 400-bp dsDNA are shown. The estimated diameters of the nanopores were 8.8 nm, 9.1 nm, 10.8 nm, 9.4 nm and 10.4 nm in order from the top to the bottom. (b) Magnified views of typical ionic-current blockades caused by SA-labelled 400-bp dsDNA passing through a nanopore. (c) Frequency ratios of current-blockade events derived from SA-labelled and non-labelled dsDNA passing through nanopores at nine different mixing ratios of SA-labelled and non-labelled dsDNA.

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