Figure 5 | Scientific Reports

Figure 5

From: Annurca apple polyphenol extract selectively kills MDA-MB-231 cells through ROS generation, sustained JNK activation and cell growth and survival inhibition

Figure 5

APE-induced ROS activate JNK/c-Jun signaling and downregulate Dusp-1 in MDA-MB-231 cells. (a) MDA-MB-231 cells were incubated with APE 100 and 300 μM EqC for 24 h. Cell lysates were prepared and analyzed by western blotting for the expression of phospho-JNK, JNK, Dusp-1, phospho-c-Jun and c-Jun. (b) Cells were treated with 300 μM EqC APE for 24 h with or without 1 h pretreatment with 5 mM NAC or 5 µM SP600125. Levels of phospho-JNK, JNK, Dusp-1, phospho-c-Jun and c-Jun were analyzed by western blotting. All results were obtained from at least three independent experiments. The full-length blots are included in the supplementary information (Figs S6, S7).

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