Figure 6 | Scientific Reports

Figure 6

From: Refinements of LC-MS/MS Spectral Counting Statistics Improve Quantification of Low Abundance Proteins

Figure 6

Analysis of expression levels of the MAI-refined DEPs. (a) A plot of EST abundances of 21 MAI-refined DEPs (ACOX3, PTGES2, HSD17B12, ACADVL, ACSL1, SUCLA2, IDH3A, ATP5C1, ATP5L, ALDH7A, HIBCH, ACAD8, SPR, GSS, PPAT, HSPA8, GMPPA, GMPPB, UGP2, RRM2 and SDHB) with selected high-abundance DEPs (PKM, GAPDH, HSP90AB1, EEF2 and HYOU1) as a reference group (SC > 100 and PLGEM-STN p-value < 0.01). (b) Western blot analysis of GMPPA, RRM2, MAVS, SOD1 and IPO4 expression levels in MDA-MB468 cells grown under the HG and GD conditions and measured relative abundance of GMPPA, RRM2, MAVS, SOD1 and IPO4 calculated from SC. Full-length blots are presented in Supplementary Information S2.

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