Figure 2

Metabolic flux ratios of intermediates in A. vinelandii wt and AV3 strain with and without ammonium. Ser from glycolysis and pyr from ED pathway are calculated using 100% 1-¹³C glucose. Other flux ratios are calculated from 20% U-¹³C glucose and 80% natural glucose. Data represent the mean of two replicates, and error was also estimated. Abbreviations: E4P, erythrose-4-phosphate; Gly, glycine; Mal, malate; OAA, oxaloacetate; OPP, oxidative pentose phosphate pathway; P5P, pentose-5-phosphate; PEP, phosphoenolpyruvate; Pyr, pyruvate; Ser,serine; ED, Entner-Doudoroff pathway; EMP, Embden-Meyerhof-Parnas pathway; GOX, glyoxylate shunt; TCA, tricarboxylic acid cycle; TKT, transketolase.