Figure 4

Detection of the amyloid beta and phosphorylated (p)-tau burden in the hippocampus of APP/PS1 mice by immunocytochemical staining. (A) APP/PS1 mice, (B) APP/PS1 mice treated with plasmon-activated water (PAW). Triple staining was performed using 4% paraformaldehyde fixation. Thioflavin-S was used to detect amyloid plaque. Protein levels of p-tau were detected by an AT8 monoclonal antibody and Alexa 594-conjugated secondary antibody. The green channel represents thioflavin-S staining, the red one represents p-tau, and the blue channel represents DAPI staining.