Table 2 Results from gene expression analysis of synovial fibroblasts stimulated with triiodothyronine (T3).

From: A thyroid hormone network exists in synovial fibroblasts of rheumatoid arthritis and osteoarthritis patients

Symbol

Description

fold change

p-value

Accession number

SNORA22

Small nucleolar RNA SNORA22

2.57

0.008

ENST00000362701

MIR199B

microRNA 199b

2.38

0.007

NR_029619

TLR4

toll-like receptor 4

1.70

0.004

NM_003266

PWRN1

Prader-Willi region non-protein coding RNA 1

−1.53

0.009

NR_026646

SLC1A6

solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6

−1.57

0.004

NM_001272087

  1. A candidate gene is given in the table when the following criteria were fulfilled:
  2. (1) Gene or nucleotide must exist in a database (accession number available).
  3. (2) mRNA of pseudogenes, mRNA with undefined genome locations, and uncharacterized mRNA were excluded.
  4. (3) The mean fold change of mRNA between control and T3 stimulation needed to be more than ±1.5-fold.
  5. (4) The p-value for the comparison should be p < 0.01 (which is a weak p-value given the thousands of genes).
  6. Positive (negative) values in mean fold change indicate higher (lower) mRNA expression of respective genes in cells stimulated (inhibited) with T3 compared to control. Whole RNA was isolated from synovial fibroblasts of 3 individual RA patients stimulated with T3 and synovial fibroblasts not stimulated (controls). Isolated whole RNA was analyzed with the Affymetrix GeneChip Human Gene 2.0 ST array.
  7. Abbreviations: MIR, microRNA; NM, NCBI Reference Sequence for genes; NR, NCBI Reference Sequence for microRNA; RA, rheumatoid arthritis; TLR, toll-like receptor.
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