Figure 5

Screening candidate R2R3-MYB genes related to the biosynthesis of WSPs in D. officinale by qRT-PCR. (A) Five stages of D. officinale stems were used to analyze gene expression. (B) Polysaccharide content in the stems of five stages. DW, dry weight. (C) Candidate genes identified using qRT-PCR. Details pertaining to S1-S5 can be found in the materials and methods and results sections. Bars represent mean ± SD (n = 3). Three biological replicates were performed. Different letters in each bar are significantly different at P < 0.05 (Duncan’s multiple range test).