Figure 4 | Scientific Reports

Figure 4

From: Application of a chemical probe to detect neutrophil elastase activation during inflammatory bowel disease

Figure 4

Detection of NE activity in oral cancer tissues. (A) Lysates prepared from normal mouse tongues or HSC-3 oral cancer tissues were labeled with 1 µM PK105b and analyzed by in-gel fluorescence (n = 3–5). *Indicates high-molecular weight species of unknown identity. (B) Total NE expression in the samples in (A) was measured by immunoblotting with an NE-specific antibody. (C,D) Densitometry analysis of active NE in (A) and total mature NE in (B), respectively. (E) Ratio of active to total mature NE in the samples. Error bars are shown as mean ± SEM. (F) NE labelling in cancer tissue was confirmed by immunoprecipitation with an NE-specific antibody. (G) Competition of PK105b labeling in oral cancer tissue lysate with PK101.

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