Figure 1

Effect of Pm-clathrin coat AP17 silencing and chlorpromazine on WSSV propagation. (A) Immunofluorescent staining analysis of Pm-clathrin coat AP17 and WSV477 in hemocytes by confocal laser scanning microscopy. Hemocytes from unchallenged and WSSV challenged were collected at 24 hpi and probed by anti-Pm-clathrin coat AP17 and WSV477 antibodies. Pm-clathrin coat AP17 and WSV477 proteins were visualized by secondary antibodies conjugated with Alexa Fluor 488 (green) and Alexa Fluor 568 (red), respectively. Nuclei were stained in blue. (B) The evaluated replicativity of WSSV in Pm-clathrin coat AP17 silencing and chlorpromazine pre-treated shrimp. Shrimp were divided into four groups and injected with either 150 mM NaCl, GFP dsRNA (10 µg/g shrimp), Pm-clathrin coat AP17 dsRNA (10 µg/g shrimp) or chlorpromazine (0.25 µg/g shrimp). After WSSV infection, the hemocyte were collected and used for determination of WSSV replicativity. The mRNA expression levels of WSSV IE1 were analyzed by Quantitative Real-time RT-PCR. The data are shown as the mean ± standard deviation. An asterisk represents significant differences from control group (p < 0.05). The experiment was carried out in triplicates.