Figure 6

Cerebral blood flow (CBF) in mice with ECM treated with artesunate plus L-arginine or saline. CBF (measured as arbitrary perfusion units, APU) in mice with ECM treated with artesunate (AS) 32 mg/kg, intraperitoneally and either L-arginine (L-ARG) 50 mg/kg or vehicle (saline – SAL). Mice were anesthetized with urethane, kept at 36 °C, had the skull exposed and were subjected to LSCI measurements of CBF. Measurements were made in separate groups of animals 1 hour, 3 hours and 6 hours after treatment, and shown as bars in the figures (n = 4–8 mice per group). The mean ± standard deviation of CBF of uninfected control mice (UNINF) or mice with ECM untreated are shown as dotted lines (mean) and coloured horizontal boxes (standard deviation; pale green for uninfected controls and pale red for mice with ECM), to allow comparison throughout the timepoints. Comparisons were made between the treated groups (SAL versus L-ARG) at each timepoint. Mice treated with artesunate plus L-arginine showed higher CBF than mice treated with artesunate plus saline at 1 hour (p = 0.0262). Data are presented as mean ± standard deviation.