Figure 1 | Scientific Reports

Figure 1

From: The Human Papillomavirus (HPV) E1 protein regulates the expression of cellular genes involved in immune response

Figure 1

HA-HPV E1 expression in HaCaT cells. (A) HPV16, 18 and 11 E1 mRNA expression. HaCaT cells transfected with the pCA control vector and the HPV E1 expressing plasmids were collected 24 h after transfection. Then RNA was extracted, cDNA synthesized, and PCR performed to evaluate E1 mRNA expression. Amplification of a fragment of the E1 mRNA indicates that all three plasmids were successfully expressed in HaCaT cells. (B) HA-tagged HPV E1 protein expression. HaCaT transfected cells were collected and protein expression ascertained by western blot using anti-HA antibody. HPV16, 18 and 11 E1 HA tagged proteins were detected at 72 KDa. Additional bands are part of the E1 protein processing. Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) was used as a loading control (37 KDa).

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