Figure 6
The HPV16, 18 and 11 E1 proteins alter similar genes. (A) Venn diagram summarizing the shared number of genes overlapping between differentially expressed genes altered by HPV E1 proteins. (B) HaCaT cells transfected with pCA vector control and HPV16 and HPV18 E1 expressing plasmids were collected and RNA extracted 24 h after transfection. Then, cDNA was synthesized and the mRNA levels of FOSB was measured using specific primers. Relative mRNA levels of FOSB showed an increase in presence of E1 from HPV16 and 18. Values are expressed as the difference in double delta-Ct compared with pCA control transfected cells. The expression of the housekeeping gene 18S was used for normalizing. Bars represent the mean ± SD. *p < 0.05 vs control (pCA).
