Figure 8
The E1 protein from HPV16, 18 and 11 downregulates interferon-stimulated genes expression. HaCaT cells transfected with pCA vector control and HPV E1 expressing plasmids were collected and RNA extracted 24 h after transfection. Then, cDNA was synthesized and the mRNA levels of CCL5, RSAD2 (Viperin) and IFIT2 genes were measured using specific primers (Supplementary Table 1). Relative mRNA levels of CCL5, RSAD2 (Viperin) and IFIT2 genes showing a decrease in presence of the HPV-16, 18 and 11 E1. Values are expressed as the difference in double delta-Ct compared with pCA control transfected cells. The expression of the housekeeping gene 18 S was used for normalizing. Bars represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs control (pCA).
