Figure 3

Cell surface markers on mucosal-associated invariant T (MAIT) cells in peripheral blood mononuclear cells. Flow cytometry was used to determine the proportions of CD69⁺ cells (A), programmed death 1 (PD-1)⁺ cells (B), T-cell immunoglobulin and mucin domain 3 (TIM-3)⁺ cells (C), and lymphocyte activation gene-3 (LAG-3)⁺ cells (D) among total MAIT cells in healthy controls (HC) (n = 28) and sarcoidosis patients (SA) (n = 40). Proportions of CD69⁺ cells and PD-1⁺ cells were significantly higher in SA. (E) Correlation of mean fluorescence intensity (MFI) of PD-1 on MAIT cells with the proportion of MAIT cells among Lineage⁻ (CD1a⁻, CD11c⁻, CD14⁻, CD19⁻, CD34⁻, CD123⁻, CD303⁻, T cell receptor γ/δ⁻, and FcεR1α⁻) T cells from SA. PD-1 MFI of MAIT cells was inversely correlated with the proportion of MAIT cells (r = −0.506, P = 0.004). *P < 0.05.