Figure 3

Scheme of the set-up and evaluation of automated HTR detection combining magnetometer and piezo sensor signal analysis. (A) Depiction of the set-up employed for the simultaneous recording of the magnetometer and piezo sensor signals. (B) Upper panel, traces corresponding to an HTR event; lower panel, traces corresponding to a jumping event recorded by both systems simultaneously. (C) Matching analysis scheme for events detected by the automated HTR detection system and the piezo sensor in control mice (n = 3) exhibiting HTR induced by DOI (1 mg/kg, i.p.). (D) Graphical depiction of the HTR detector false positive detection rate (FDR) when used alone, or in combination with piezo sensor signal maxima matching, in mice (n = 3) exhibiting jumping behavior during naloxone (10 mg/kg, i.p.)-precipitated withdrawal (morphine 50 mg/kg, i.p., 4 h prior).