Figure 4
From: Fully automated head-twitch detection system for the study of 5-HT2A receptor pharmacology in vivo
Time-dependent effect on HTR and disposition profile of DOI. (A) HTR induced by DOI (1 mg/kg, i.p.) along with time after the administration of the drug (n = 5) or vehicle (n = 4). (B) Temporal distribution of DOI (1 mg/kg, i.p.) in mouse whole blood and forebrain (n = 4 per time point and tissue). (C) HTR time-course on mice treated with DOI (1 mg/kg, i.p.) put under isoflurane anesthesia (1.5%, n = 10) or held in the home cage (n = 9) for the first 30 min following administration of the drug. (D) Quantification of total HTR count between 60–150 min. Two-tailed Student’s t-test (D). Values represent mean ± S.E.M. (n.s., not significant). Arrow indicates time of administration of DOI or vehicle.
