Figure 2
Genomic comparative analysis of 21 collected mastitis isolates, allocation of the RsbU mutation and asp23 RT-qPCR. (A) Each circle represents one isogenic variant (Variant 1 = V1 etc.), based on the strains SNP difference, where the size of the circle relates to the number of isolates carrying it and the distance of the circles to each other corresponds to the number of SNP(s). The number of isolates collected at a specific week (W1–14) are shown in parentheses (one isolate at the 1st week, W1(1) etc.). See Table S1 for details. v, variants; w, week of sampling; IN, initial isolate; HA, host-adapted isolate. (B) Genetic loci encoding for SigB expression in S. aureus. The grey area in the rsbU gene represents the gene sequence in which the SNP (G368A) of the HA isolate was identified. The corresponding protein-missense mutation (G122D) is indicated in red in the RsbU cartoon. (C) Asp23 expression of strains grown in TSB to an OD 600 nm of 2.5 and 5.0 was evaluated by RT-qPCR. The data shown are the means of three independently grown strains and their technical duplicates. Statistical difference between the expression of the initial and host-adapted isolate was determined with unpaired Students t test on log10 transformed data. (***P < 0.001). IN, initial isolate; HA, host-adapted isolate.
