Table 1 Gene expression analysis. Primer sequences and Universal Probe Library probe numbers used for q-RTPCR experiments to determine gene expression levels in aortic tissues.
Gene | Primer | Sequence (5′-3′) | Universal Probe Library (Roche) Probe no. |
|---|---|---|---|
Il6 | Sense | GCTACCAAACTGGATATAATCAGGA | 6 |
Anti-sense | CCAGGTAGCTATGGTACTCCAGAA | ||
vacm1 | Sense | AACAACCGAATCCCCAACTT | 83 |
Anti-sense | TGATTGGGAGAGACAAAGCA | ||
mcp-1 | Sense | GATCATCTTGCGGTGAATGAGT | 62 |
Anti-sense | CATCCACGTGTTGGCTCA | ||
tnf-α | Sense | TTGAGATCCATGCCGTTG | 25 |
Anti-sense | CTGTAGCCCACGTCGTAGC | ||
gapdh | Sense | AATCTCCACTTTGCCACTGC | GAPDH Assay Probe (Roche) |
Anti-sense | ATGGTGAAGGTCGGTGTGA |
