Figure 1 | Scientific Reports

Figure 1

From: Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)

Figure 1

Feeding RNAi in mealybug with 380 µg of dsRNA imbibed in leaf using petiole dip assay. (A) Experimental setup for mealybug feeding. (B) Uptake and stability of dsRNA extracted from leaf visualized on agarose gel (1%) Lane1: Crude dsGFP (+ve control) Lane 2: dsRNA isolated from the leaf after uptake. Relative expression of bursiconand IAP in Phenacoccus solenopsis 48 h post feeding in comparison to GFP control: (C) Bursicon (54.1% knockdown) (D) IAP (59.4% knockdown). The expression level of each gene has been normalized with 28 s as internal control. The error bars represent the standard deviation (3 replicates) and *represents significant differences in mRNA transcripts compared to GFP control (P ≤ 0.05, Student’s t-test).

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